Exploration of Global Gene Expression Patterns in Pancreatic Adenocarcinoma Using cDNA Microarrays
نویسندگان
چکیده
منابع مشابه
Exploration of global gene expression patterns in pancreatic adenocarcinoma using cDNA microarrays.
Pancreatic cancer is the fifth leading cause of cancer death in the United States. We used cDNA microarrays to analyze global gene expression patterns in 14 pancreatic cancer cell lines, 17 resected infiltrating pancreatic cancer tissues, and 5 samples of normal pancreas to identify genes that are differentially expressed in pancreatic cancer. We found more than 400 cDNAs corresponding to genes...
متن کاملExpression profiling in cancer using cDNA microarrays.
Currently there are over 1,000,000 human expressed sequence tag (EST) sequences available on the public database, representing perhaps 50-90% of all human genes. The cDNA microarray technique is a recently developed tool that exploits this wealth of information for the analysis of gene expression. In this method, DNA probes representing cDNA clones are arrayed onto a glass slide and interrogate...
متن کاملPATTERNS OF GENE EXPRESSION IN SUGARCANE MONITORED USING cDNA MACROARRAYS
A sugarcane Expressed Sequence Tag (EST) database has been established which has led to the identification of genes in different tissues. Gene identity alone, however, does not provide information about patterns of gene expression nor allow elucidation of gene function. As a first step towards functional genome analysis in sugarcane, expression profiles are being developed for a random selectio...
متن کاملUncovering evolutionary patterns of gene expression using microarrays.
The advent of microarray technology is providing new insights into fundamental questions in evolutionary biology. Here, we review the recent literature on the use of microarrays to study the evolution of genome-wide patterns of gene expression within and between species. Large levels of variation in gene expression patterns have been observed at the intra and interspecific level, and a substant...
متن کاملIndirect measurements of differential gene expression with cDNA microarrays.
The use of universal RNA reference sets is an increasingly common approach to molecular classification studies with cDNA microarrays. Here we evaluated the reliability of indirect measurements of fluorescence ratios with a common RNA reference as a means of identifying differentially expressed genes. Comparisons of direct and indirect measures of differential gene expression showed a strong ove...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The American Journal of Pathology
سال: 2003
ISSN: 0002-9440
DOI: 10.1016/s0002-9440(10)63911-9